The results of an object analysis are output in the Count and Measure Results tool window. Use the Options > Count and Measure > Results dialog box to make general settings for the output of results. You can find an overview of the Options > Count and Measure dialog box here.
Description of the function groups
Accumulation in grid and histogram
See also
Overview - Automatic image analysis
Grid
Outputting the results for several images
Select the Only show results of the active image check box if, in the Count and Measure Results tool window, you only want to show the results for the image that is active in the document group at the moment.
When the Only show results of the active image check box hasn't been selected, the results for all of the analyzed images are displayed together in the document group. By using the Image measurement parameter, you can allocate the results unambiguously to the corresponding image.
Outputting the results for multi-layer images
The Only show results of the active image check box is only relevant when you're performing an image analysis on a multi-layer image.
Outputting the results for image series
In an image series, several frames have been combined into one single file. Movies are an example of an image series. An object analysis will be performed for every frame in an image series. Select the Only show results of the active frames check box if you want to show only the results for the frame of the image series that is currently on display in the image window.
You can adopt the number of the analyzed frame in the results sheet. To do this, in the Options > Count and Measure > Measurements dialog box, click the Select Object Measurements button to open the Select Object Measurements dialog box. From the Available Object Measurements list, select the Index t (Value t) parameter. The frame's index will then be shown in the Index column in the results sheet.
When the Only show results of the active frames check box is not selected, the results for all of the frames of an image series are displayed together on one sheet.
Note: The Only show results of the active frames setting applies only to the display of the results, and not to the analysis itself. Whether or not, all of the frames in an image series are to be analyzed, you decide in the Options > Count and Measure > General dialog box, located in the Target group.
Outputting the results for ROIs
You can also limit an object analysis to interesting areas of the image, so-called ROIs (Region of Interest). Select the Show class results per ROI when the class results are to be output per ROI.
You can show the names of the ROIs in the Object Measurements, Class Histogram, ROI Measurements, and ROI Histogram results views. To do this, in the Options > Count and Measure > Measurements dialog box, click the Select Object Measurements button to open the Select Object Measurements dialog box. From the Available Object Measurements list, select the ROI parameter. The ROI's name will then be shown in the results views mentioned above, in the ROI column.
If the Show class results per ROI check box hasn't been selected, the class results for all of the ROIs that have been defined on the image will be accumulated in the Class Measurements results sheet.
Example: You have defined 3 ROIs on an image. The Show class results per ROI check box has not been selected. In this case, only one result will be shown for all 3 ROIs, in the Class Measurements results sheet. When you select the Show class results per ROI check box, the class results for each ROI will be output separately in the Class Measurements results sheet.
Grid & Histogram
Objects that, owing to the current settings, don't fall into any of the defined classes, will be automatically assigned to a class with the index 0.
Select the Show unclassified class check box if you want to show the results of class 0 in the Class Measurements and Class Histogram results views of the Count and Measure Results tool window.
Note: In the image, the unclassified objects are displayed regardless of the status of the check box. Unclassified objects appear cross hatched in the image.
Accumulation in grid and histogram
The setting in this group is only relevant when you're working with time stacks. A time stack could be a movie that you acquire in the Camera Control tool window or an AVI file that you load in your software.
Select the Accumulate class and ROI results over time check box, when the classes and ROI results for all of a time stack's images are to be combined in the Class Measurements results sheet.
If this check box hasn't been selected, the results in the sheet and in the class histogram will be output for every frame in the time stack. You can adopt the numbers of the frames in the results sheet. To do this, in the Options > Count and Measure > Measurements dialog box, click the Select Object Measurements button to open the Select Object Measurements dialog box. From the Available Object Measurements list, select the Value t parameter. With Index t the numbers of the frames, and with Value t, the acquisition time, will be specified.
Note: If you want to use the Accumulate class results over time function, the Only show results of the active frames check box must be clear. When both check boxes have been selected at the same time, the results for the image series' currently active frame will always be shown.
Example: You define a phase on a time stack with 100 frames and perform an image analysis. Apply the Phase classification scheme so that all of the objects fall into a single class.
Make sure that in the Options > Count and Measure > General dialog box, the All frames and channels option has been selected.
Case 1: In the Grid group, clear the Only show results of the active frames check box, and clear the check box in the Accumulation in grid and histogram group.
The Class Measurements results view now contains 100 results. You see the results at every time point.
In this case, the histogram in the Class Histogram results view shows the results for the frame that is currently on display on your monitor. Use the navigation bar in the image window to have other frames displayed. The histogram will be updated correspondingly.
Case 2: Now, select the Only show results of the active frames check box. Perform the analysis once more, with the altered settings. To do so, click the Count and Measure button located in the Count and Measure tool window.
In this case, the Class Measurements results view always contains only one individual result, for the frame that is at that moment active. Use the navigation bar in the image window to have another frame displayed. The values for the image you've selected will be correspondingly updated in the results sheet and in the chart.
Case 3: In the Grid group, clear the Only show results of the active frames check box and mark the check box in the Accumulation in grid and histogram group. Perform the analysis once more, with the altered settings. To do so, click the Count and Measure button located in the Count and Measure tool window.
In this case, the Class Measurements results view always contains only one consolidated result, accumulated for all images. The histogram also displays the consolidated result.
Statistics
When an object analysis is performed, a series of measurements will be carried out. You set the measurement parameters that you want to adopt in the results sheet, in the Select Object Measurements dialog box. You can open the dialog box by clicking the Select Object Measurements 
button located in the Count and Measure Results tool window. The results will be output in the Count and Measure Results tool window, in a results sheet. For each selected measurement parameter can, in turn, statistical parameters be calculated.
The statistical parameters will be output beneath the column for the respective measurement. For some parameters, for example Document, no statistical parameters can be output. In this case, a dash will be shown in the corresponding cell.
The statistical values will be shown in the following results views:
Possible statistical parameters
You can choose between various statistical parameters.
Select the Count check box if the number of individual measurements that have gone into the respective statistical parameters, for example into the mean value for the area, are to be shown. This depends, among other things, on the number of phases that have been defined and the number of frames that have been included in the measurement.
For example: You have analyzed a time stack with 10 frames and two defined phases. The Only show results of the active frames check box has been cleared. For every measurement parameter, the value 20 will stand in the Count field. That means that on all 10 frames, a total of 20 objects were found and measured. The objects concerned can belong to either the phase 1 or the phase 2.
Select the Count in filter ranges check box when only the measurement results within the currently defined filter range are to be shown.
Should no filter range have been defined, this value will be the same as the total number of objects that were measured. You can find more information on how to define filter ranges for objects here.
Select the Minimum check box when for each measurement parameter the smallest measurement value is to be shown.
Select the Maximum check box when for each measurement parameter the greatest measurement value is to be shown.
Select the Mean check box when for each measurement parameter the mean value is to be output.
Select the Standard Deviation check box if for every measurement parameter the standard deviation (deviation from the mean value) is to be output.
Select the Sum check box when the measurement results are to be added up. The complete area that is covered by the current phase definition is arrived at, for example, by adding up the object areas.
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