Use the Define 'One parameter' Classification dialog box to define a classification scheme for detected objects. This classifies objects in an image on the basis of a chosen measurement parameter, the area for example. You can, for example, define several size classes for objects. This enables you to determine the size distribution of the objects in the image.
Opening the dialog box
1.You can open this dialog box by clicking the small black arrow next to the Count and Measure button, located in the Count and Measure tool window.
2.From the button's context menu, select the Classification Options... entry to switch to the Options > Count and Measure > Classification dialog box.
3.There, click the New Classification 
button, then select the New 'One parameter' Classification entry.
The dialog box's structure
The dialog box is made up of several groups. Click on one of the areas in the dialog box shown above to jump to the corresponding help topic.
(1) Assigning the classification a name
(2) Selecting measurement parameters
(5) Performing a classification
See also
Overview - Automatic image analysis
Carrying out an automatic image analysis
(1) Assigning the classification a name
Enter a descriptive name for your classification scheme in the Name field. This name will be adopted in the Options > Count and Measure > Classification dialog box's Current classification list and can then be selected for an automatic image analysis.
(2) Selecting measurement parameters
Using the picklist
Use the Measurement picklist to choose a measurement parameter by which the objects are to be classified.
Note: Only measurement parameters that have already been entered in the Measurements computed for all objects list, located in the Select Object Measurement dialog box, may be used.
Example: Your image contains round and elongated objects. You want to know how many objects are round and how many are elongated. In this case, select the Shape Factor measurement parameter from the Measurement list.
With the Shape Factor measurement parameter, you can sort the round and elongated objects into different classes.
Adding measurement parameters that are missing
Should the required measurement parameter not yet be offered in the picklist, click the Select Object Measurements button to switch to the Select Object Measurements dialog box. There, you can add the required measurement parameter. You can find more information on this dialog box here.
Selecting a unit
Click this button 
to specify the unit of measure for the selected measurement parameter. The button will only appear in the dialog box when the selected measurement parameter has also been linked to a unit. Some parameters, Shape Factor for example, have no unit.
Select the basic unit from the Unit menu. The button will then display the basic unit you've selected. Click the button again, and open the Prefix menu. Select the appropriate entry. The button's label will be changed correspondingly.
The selected unit has an effect on all of the values in the dialog box: On the X-values in the histogram, and the limit values for the individual classes. The unit that has been chosen here, doesn't have an effect on the values in the results sheets.
(3) Defining object classes
Use the Object classes definition group to specify the number and the names of the classes. You also define the class limits for each class here.
Adding classes
Click the Add Object Class button to add another object class.
Double click the field in the Class Name column to enter a name for the class.
Double click the field in the Color column to open the color palette and to select a color for the class. The class will be displayed in the color you have assigned it, in the image window and in the histogram.
The class name and color are also displayed in the results views in the Count and Measure Results tool window.
Deleting classes
Click the Remove Object Class 
button to delete a class. At least one class will always be defined. It's only possible to remove a class when at least two classes have been defined.
Adopting class limits as the class name
Click the Set Class Names to '[Min. ;Max.]' 
button to use the class limits as class names. The values in the [Min. and Max.] fields are automatically adopted in the Class Name field. The class limits are then also shown as class names in the results sheet. For the class results, select the Class Name parameter to have the name of the class shown for every measurement value in the results sheet.
Automatic classification
As a rule, you calculate the class limits automatically. Click the Automatic Classification 
button to perform an automatic classification of the objects. When an automatic classification is made, the class limits will be automatically calculated. If an automatic object analysis has already been performed on the active image, you can use the upper and lower values of the selected measurement parameter as a class limit. These class limits can still be edited afterwards.
Click the Automatic Classification button to open to the Automatic Classification dialog box. You can find more information on this dialog box here.
Defining class limits
In the [Min. field, the lower classification limit will be specified. In the Max.[ field, the upper classification limit will be specified. The upper value itself no longer belongs to the class. The value in this case, is always based on the measurement parameter that has been selected in the Measurements list, and the corresponding unit. That's to say, a value of 100, corresponds with an area of 100 µm², when the Area measurement parameter and the µm² unit have been chosen.
Double click in the [Min., field to enter a lower value for each of the classes. You can either edit the value directly, or select it via the arrow keys. Enter the upper value in the Max.[ field in the same way.
Should very small or very large values be specified here, select another unit of measurement. For example, with very large areas switch from the µm² to the mm². You can then more easily edit the values.
It is also possible to cumulate the values by not setting a lower or upper limit.
Click the Clear minimum value 
button, when no lower limit is to be defined for the classification.
Click the Clear maximum value 
button, when no upper limit is to be defined for the classification.
(4) Histogram display
The histogram here shows the distribution of the chosen measurement parameters in the image.
The histogram shows the distribution of the small and large objects. One can see that there are far more small objects (blue histogram area), than large ones, (yellow histogram area), in the image.
Setting up class limits via the histogram
The class limits can also be easily set up by using the histogram.
In the image, the class limits haven't yet been set very well. Too many large objects have been allocated to the small object class (blue).
By using the histogram, you can customize the class limits.
1.To do this, move your mouse over the histogram, then click the area, (here yellow or blue), whose class limits you want to edit.
2.Then move your mouse to the separation line between the class areas.
3.The mouse alters its appearance and changes into a double arrowhead.
3.Keep the left mouse button pressed.
4.The classes are right next to one another. Decrease one class in order to increase the adjacent class. You can move the range in such a way that all of the values that should fall into the corresponding class are included.
4.Check your settings, by clicking the Count and Measure button.
5.The changed class limits will then be correspondingly colorized in the image.
The class limits are adjusted, the separation between small and large objects is now successful.
Changing the histogram display
Use these buttons to change the way the histogram is displayed. Click here to learn more.
(5) Performing a classification
Click the Count and Measure or Count and Measure on ROI button to apply the current classification scheme. This gives you the opportunity to test the classification, and, if necessary, to adjust it.
Now click the Close button to exit the dialog box.
Click the OK button to save the current values, without performing the classification.
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