Your software offers a wide range of measurement functions. They enable you to quickly count objects and measure segments and areas. All the results will be saved together with the image and can also be issued as a sheet.
Selecting the measurement environment
Displaying and saving measurement results
Measuring on different image types
Prerequisite
For making measurements, correctly calibrated images are an essential prerequisite.
If your transmission electron microscope's microscope magnification can be read via remote control, then images acquired with your software are automatically calibrated. The actual magnification of the acquired images depends on the camera's position and may differ from the set microscope magnification. Use the Magnification calibration process to acquire correctly calibrated images. You can find more information on this topic here.
Should the image not yet have been calibrated, use the Image > Calibrate Image... command to carry out a calibration.
See also
Selecting the measurement environment
Measuring with help of the tool window
Switch to the Processing layout when you want to measure images. You can find the Measurement tool window in the bottom section of this layout. In this tool window you have fast access to all measurement functions and settings which relate to the measurement. This tool window is at the same time the measurement display and contains all of the values that have been measured on the active image.
Note: Should, right at the bottom of the user interface, several tool windows lie one over the other, activate the Measurement tool window, by clicking on the header of the 
Measurement tab. The tabs can be found under the tool windows.
Measuring with help of the toolbar
Should you need more room for displaying the image, you can also use the Measurement toolbar instead of the tool window. All the measurement functions can also be found on the Measurement toolbar. To have this toolbar displayed, use the View > Toolbars > Measurement command. Begin a measurement by simply clicking the appropriate button. In this case you will only see the measurements that you have carried out in the image.
Measuring with help of menu commands
The Measure menu also contains all of the measurement functions. To start a measurement, simply use the corresponding menu command. In this case you will only see the measurements that you have carried out in the image.
Starting a measurement
Begin a measurement by selecting the measurement function you want. You can find the measurement function in the Measurement tool window, on the Measurement toolbar, or in the Measure menu.
Working in the measurement mode
As soon as you have clicked a measurement function, your software will automatically switch to a measurement mode. In the measurement mode your mouse pointer will take on the shape of a cross on the image. You can make as many measurements as you like with the measurement function that has been selected. The continuous measurement mode is valid for all loaded images. You can, therefore, easily measure numerous images one after the other.
The selected measurement function's button will keep its clicked appearance and in this way show you the current measurement function. You can recognize this status by the button's background color.
Finishing the measurement mode
You will remain in this measurement mode until you explicitly switch it off. To do so, click the Select Measurement Objects 
button. You can find the button either in the Measurement tool window or on the toolbar.
Changing the default measurement mode
The continuous measurement mode described above is preset by default. You can change this default setting. To do this, use the Tools > Options... command. Select the Measurement > General entry in the tree view. Select the Switch to 'Select Measurement Objects' mode after creating a measurement object check box. Then, when you have completed a measurement, you will automatically leave the measurement mode again. This means you have to select the measurement function again before you start each interactive measurement.
Displaying and saving measurement results
The measurement results will be displayed directly on the image and in the Measurement tool window. Should this tool window not be visible, use the View > Tool Windows > Measurement command to bring up the tool window.
Saving the measurement results
The measurements will be saved along with the image, if you save the image in the TIF or VSI file format. You can, however, also export the measurement results in a results sheet, and save this as a file.
Showing and hiding measurement results in an image
The measurement results will be shown on the image in a special data layer, the measurement layer. On your monitor, image and measurement layer are shown together. The data of each, however, is individually stored if you use the TIF or VSI image file format. Try and picture the measurement layer as a transparency which is placed over the image. When you measure an image, the image data will not be changed by having the measurement results displayed on it.
You can, at any time, hide or show the measurement layers.
To do so, use the Layers tool window. There you have access to all of an image's layers. The eye icon 
identifies all of the layers that are currently on display on your monitor.
Click the eye icon in front of the measurement layer to hide the measurements. Click an empty cell without an eye icon to make the corresponding layer reappear.
Setting the unit for the measurement results
The unit in which the measurement results will be issued, is determined by the measurement function that has been selected and the image's calibration. But you can choose whether the length e.g., is shown in mm or Angström.
Use the Tools > Options... command. Select the Measurements > Results entry in the tree view. Select the unit you want to use from the Prefix of the unit list.
Outputting measurement results in a sheet
You can export the measurement results from the Measurement tool window as a sheet, for example, to be able to save the measurement results in their own file, independently of the image. You will find the functions, e.g., as a button next to the measurement functions on the Measurement tool window's toolbar.
Click the Export to Excel 
button to export the results to a MS-Excel sheet. Use this export possibility, for example, when you want to evaluate the measurement results still further. This will also enable you to supply the results to other users who don't have your software.
Editing measurements
You can edit existing measurement objects at any time. The measurement values in the Measurement tool window will then be correspondingly updated. To do so, first click the Select Measurement Objects button, and then select the measurement object(s) you want to edit. You can find the button either in the Measurement tool window or on the toolbar.
Note: When you load an image file with measurement objects, it is only possible to edit the measurement objects if the image file has been saved in the TIF or VSI image file format.
Moving measurement objects
You can move a whole measurement object while keeping the left mouse button pressed.
Increasing/decreasing the size of measurement objects
You can also change the size of a measurement object. Move the pointer onto a marker. By dragging the marker with the mouse button depressed, you can adjust the frame's size as wished.
Change the measurement object by moving the handles.
Deleting measurement object
Click the [Del] key on your keyboard in order to delete the selected measurement object.
Changing the color, font, and line thickness of individual measurement objects
You can, at any time, change the color, font, and line thickness, of individual measurement objects. Select one or more measurement objects in an image and click your right mouse button to open a context menu. In the context menu you'll find several commands with which you can change the appearance of the selected measurement objects.
From the context menu, select, e.g., the Change Color... command. Select the color you would like from the color palette, then close the dialog box with OK.
Measuring in the live mode
All of the measurement functions are also available in the live-image. You can therefore, e.g., quickly measure a segment in the live-image.
Measuring on different image types
Measuring on multi-dimensional images
You can combine a series of individual images into one image. What results is e.g., a time stack in which all of the frames will have been acquired at different times.
You can make measurements on every separate image. Display the required frame on your monitor. To do this, use the navigation bar in the image window. Then carry out the measurement on this frame. The measurement will be permanently linked to this frame, i.e., the measurement will only be displayed on your monitor when the frame on which you made this measurement is also on display.
The measurement results will be shown in the Measurement tool window. You can give every measurement the number of the frame on which it was made. To do so, use, e.g., the measurement parameter Index t for time stacks.
Measurement precision
How precise the measurement is, depends on the X/Y-calibration and the image's current zoom factor.
Influence of the X/Y-calibration
The X/Y-calibration defines the width and height of the sample area that is represented by one pixel. For example, it could be that one pixel displays a sample area of 10 µm x 10 µm. A pixel is the smallest image structure that can be measured. For this reason, the maximum measurement precision where this example is concerned, is 10 µm.
Influence of the zoom factor
The zoom factor tells you how large the image will be displayed on your monitor. With a zoom factor of 100%, one pixel on the monitor equals exactly one pixel in the image. With a zoom factor of 50%, one pixel on the monitor equals 2 x 2 pixels in the image. When you make a measurement, you should use the zoom factor 100% whenever possible. Then you will achieve a maximum of measurement precision. Should the zoom factor 100% not be possible, because the image area you want to measure can't then be completely seen, choose the largest possible zoom factor under 100%.
Information about changing an image's zoom factor can be found here.
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